Clarity™
Epstein-Barr Virus (EBV) Quantification Kit

Epstein-Barr Virus (EBV) is a double-stranded DNA γ-herpesvirus associated with different malignancies. Quantification of the EBV DNA load has shown to be beneficial for early disease diagnosis as well as accurate monitoring of treatment responses. The Clarity™ EBV Quantification Kit is developed for sensitive detection and quantification of EBV DNA using the Clarity™ digital PCR system (Cat. No. 10001). The kit detects both the single-copy Epstein-Barr nuclear antigen-I (EBNA-I) as well as the multi-copy BamHI-W gene, enabling quantitative and qualitative measurements of EBV DNA isolated from human plasma or serum.

Features

Reproducibility

Precise quantification of EBV specific DNA

Ease of Use

Low hands-on-time, compatible with most conventional thermal cyclers

High Sensitivity

Optimized to detect <0.5 copy/μL reaction

Manufactured in Singapore under ISO 13485

High Throughput Analysis

Analyze up to 96 reactions in 4 hours

Product Performance

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The accuracy and sensitivity of Clarity™ EBV quantification kit were validated by quantifying EBNA-I and BamHI-W in a duplex assay. The results showed concordance between the expected and measured concentrations of the target DNA. In addition, the system demonstrated sensitive detection to as low as 0.28 copies/µL DNA, and excellent linearity (R2>0.999) across a dynamic range from 0 – 3000 copies/µL.

Specifications

TechnologyDigital PCR
Target SequenceEBNA-I, BamHI-W
Sensitivity (LOD)< 0.5 copy /µL reaction
Time to Result< 4 h
Linear Range of Measurement0 – 3000 copies /µL reaction
Specimen TypeHuman plasma or serum samples
Reporting FormatResults reported in copies /µL
Instrument TestedClarity™ digital PCR system (Cat. No. 10001)
Additional Material RequiredClarity™ consumables package (Cat. No. 10011)
Detection ChannelsFAM (EBNA-I), HEX (BamHI-W)
Kit Storage-20°C, avoid repeated freezing and thawing of kit contents

Ordering Information

Product nameDescriptionCat. No.
Clarity™ EGFR T790M Mutation Quantification Kit48 reactions10016
Clarity™ Epstein-Barr Virus Quantification Kit96 reactions10017
Clarity™ Hepatitis B Virus Quantification Kit96 reactions10018
Clarity™ EGFR L858R Mutation Quantification Kit48 reactions10019
Clarity™ IDH2 Mutation Quantification Kit48 reactions10021
Clarity™ Major BCR-ABL Mutation Quantification Kit96 reactions10022
Clarity™ Minor BCR-ABL Mutation Quantification Kit96 reactions10023