IDH2 Mutation Quantification Kit

Isocitrate dehydrogenase 2 (IDH2), a nicotinamide adenine dinucleotide phosphate (NADP)-dependent enzyme in mitochondria, is a critical component of the tricarboxylic acid cycle and involves in diverse cellular processes including histone demethylation and DNA modification. Two most frequently detected IDH2 mutations are G419A and G515A, which results in the R140Q and R172K amino acid substitutions respectively. These recurrent somatic mutations in the arginine residues promote tumorigenesis and cell proliferation, and have been identified in multiple malignancies such as acute myeloid leukemia, angioimmunoblastic lymphomas and glioma. Hence detection of the IDH2 R140Q and R172K mutations is clinically significant for tumour diagnosis, selection of targeted treatments and monitoring of disease prognosis. The Clarity™ IDH2 Mutation Quantification Kit provides ready-to-use reagents optimized for sensitive detection and quantification of IDH2 R140Q and R172K mutant from human plasma or serum using the Clarity™ digital PCR system.



Precise quantification of IDH2 R140Q and R172K mutation abundance

Ease of Use

Low hands-on-time, compatible with most conventional thermal cyclers

High Sensitivity

Detect as low as 0.1% of IDH2 R140Q and R172K mutant DNA

Manufactured in Singapore under ISO 13485

High Throughput Analysis

Analyze up to 96 reactions in 4 hours

Product Performance


Clarity™ IDH2 Mutation quantification kit detected as low as 0.1% of IDH2 R140Q or R172K mutant DNA in a high background of 15,000 copies of wild-type IDH2. Data was plotted against the expected R140Q or R172K mutant fraction respectively, and both results showed excellent linearity from 10% down to 0.1% with an R2 value > 0.999.


TechnologyDigital PCR
Target SequenceIDH2 wild-type and R140Q or R172K mutant DNA
Sensitivity (LOD)0.1% mutation
Time to Result< 4 h
Specimen TypeHuman plasma or serum samples
Reporting FormatRatio of mutant versus wild-type target
Instrument TestedClarity™ digital PCR system (Cat. No. 10001)
Additional Material RequiredClarity™ consumables package (Cat. No. 10011)
Detection ChannelsFAM (R140Q or R172K mutant DNA), HEX (EGFR wild-type DNA)
Kit Storage-20°C, avoid repeated freezing and thawing of kit contents

Ordering Information

Product nameDescriptionCat. No.
Clarity™ EGFR T790M Mutation Quantification Kit48 reactions10016
Clarity™ Epstein-Barr Virus Quantification Kit96 reactions10017
Clarity™ Hepatitis B Virus Quantification Kit96 reactions10018
Clarity™ EGFR L858R Mutation Quantification Kit48 reactions10019
Clarity™ IDH2 Mutation Quantification Kit48 reactions10021
Clarity™ Major BCR-ABL Mutation Quantification Kit96 reactions10022
Clarity™ Minor BCR-ABL Mutation Quantification Kit96 reactions10023