EGFR L858R Mutation Quantification Kit

The T2573G mutation results in an amino acid substitution at position 858 in human epidermal growth factor receptor (EGFR), from a leucine (L) to an arginine (R). The L858R mutation accounts for the majority of EGFR-mutated non-small cell lung cancer (NSCLC), and it displays higher sensitivity to first line EGFR tyrosine kinase inhibitor therapy as compared to wild type EGFR. Therefore, monitoring L858R mutation status is crucial for the prescription of targeted therapies and prediction of their clinical responses. The Clarity™ EGFR L858R Mutation Quantification Kit provides ready-to-use reagents optimized for sensitive detection and quantification of L858R mutant DNA from human plasma or serum using the Clarity™ digital PCR system.



Precise quantification of L858R mutation abundance

Ease of Use

Low hands-on-time, compatible with most conventional thermal cyclers

High Sensitivity

Detect as low as 0.1% of L858R mutant DNA

Manufactured in Singapore under ISO 13485

High Throughput Analysis

Analyze up to 96 reactions in 4 hours

Product Performance


Clarity™ EGFR L858R Mutation Quantification Kit detected as low as 0.1% of L858R mutant DNA in a high background of 15,000 copies of wild-type EGFR. When measured L858R mutant fraction was plotted against the expected mutant fraction, the results showed excellent linearity from 10% down to 0.1% with an R2 value > 0.999.


TechnologyDigital PCR
Target SequenceEGFR wild-type and L858R mutant DNA
Sensitivity (LOD)0.1% mutation
Time to Result< 4 h
Specimen TypeHuman plasma or serum samples
Reporting FormatRatio of mutant versus wild-type target
Instrument TestedClarity™ digital PCR system (Cat. No. 10001)
Additional Material RequiredClarity™ consumables package (Cat. No. 10011)
Detection ChannelsFAM (L858R mutant DNA), HEX (EGFR wild-type DNA)
Kit Storage-20°C, avoid repeated freezing and thawing of kit contents

Ordering Information

Product nameDescriptionCat. No.
Clarity™ EGFR T790M Mutation Quantification Kit48 reactions10016
Clarity™ Epstein-Barr Virus Quantification Kit96 reactions10017
Clarity™ Hepatitis B Virus Quantification Kit96 reactions10018
Clarity™ EGFR L858R Mutation Quantification Kit48 reactions10019
Clarity™ IDH2 Mutation Quantification Kit48 reactions10021
Clarity™ Major BCR-ABL Mutation Quantification Kit96 reactions10022
Clarity™ Minor BCR-ABL Mutation Quantification Kit96 reactions10023