EGFR T790M Mutation Quantification Kit

The C2615T mutation results in an amino acid substitution at position 790 in human epidermal growth factor receptor (EGFR), from a threonine (T) to a methionine (M). Patients who develop T790M mutation acquire resistance to first line EGFR tyrosine kinase inhibitor therapy and as such, monitoring the T790M mutation status is crucial to guide treatment decisions. The Clarity™ EGFR T790M Mutation Quantification Kit provides ready-to-use reagents optimized for sensitive detection and quantification of T790M mutant DNA from human plasma or serum using the Clarity™ digital PCR system.



Precise quantification of T790M mutation abundance

Ease of Use

Low hands-on-time, compatible with most conventional thermal cyclers

High Sensitivity

Detect as low as 0.1% of T790M mutant DNA

Manufactured in Singapore under ISO 13485

High Throughput Analysis

Analyze up to 96 reactions in 4 hours

Product Performance


ClarityTM EGFR T790M Mutation quantification kit detected as low as 0.1% of T790M mutant DNA in a high background of 15,000 copies of wild-type EGFR. When the data was plotted against the expected mutant fraction, the results showed excellent linearity from 10% down to 0.1% with an R2 value of 0.9994.


TechnologyDigital PCR
Target SequenceEGFR wild-type and T790M mutant DNA
Sensitivity (LOD)0.1% mutation
Time to Result< 4 h
Specimen TypeHuman plasma or serum samples
Reporting FormatRatio of mutant versus wild-type target
Instrument TestedClarity™ digital PCR system (Cat. No. 10001)
Additional Material RequiredClarity™ consumables package (Cat. No. 10011)
Detection ChannelsFAM (T790M mutant DNA), HEX (EGFR wild-type DNA)
Kit Storage-20°C, avoid repeated freezing and thawing of kit contents

Ordering Information

Product nameDescriptionCat. No.
Clarity™ EGFR T790M Mutation Quantification Kit48 reactions10016
Clarity™ Epstein-Barr Virus Quantification Kit96 reactions10017
Clarity™ Hepatitis B Virus Quantification Kit96 reactions10018
Clarity™ EGFR L858R Mutation Quantification Kit48 reactions10019
Clarity™ IDH2 Mutation Quantification Kit48 reactions10021
Clarity™ Major BCR-ABL Mutation Quantification Kit96 reactions10022
Clarity™ Minor BCR-ABL Mutation Quantification Kit96 reactions10023